The solvent extraction of copper and zinc from the bioleaching solutions of low-grade sulfide ores with LIX984 and D2EHPA was investigated. The influences of extractant content, aqueous pH value, phase ratio and equilibration time on metals extraction were studied. The results show that LIX984 has a higher selectivity for copper than for iron, zinc and other metals, and has the copper extraction rate above 97%, while the zinc and iron extraction rate is less than 1.6% respectively. Zinc extraction is carried out following the copper extraction from the raffinate. The zinc extraction with di(2-ethylhexyl) phosphoric acid (D2EHPA) is low due to its poor cation exchange. A sodium salt of D2EHPA is used and the zinc extraction rate is enhanced to above 98%. Though iron (III) is strongly extracted before the extraction of zinc by D2EHPA, it is difficult to strip iron from the organic phase by sulfuric acid. The zinc stripping rate is above 99% with 100 g/L sulfuric acid, while that of iron is 0.16%. Hence, the separation of zinc from iron can be achieved by the selective stripping.